The InSight X3+ and InSight X3 are built on Spectra-Physics’ industry leading InSight platform. Designed for maximum performance and reliability, the InSight X3+ delivers 50% high powers across the tuning range for brighter signals and increased imaging depths. The InSight X3+ and InSight X3 lasers have the largest installed base with field proven reliability. As a result, researchers have generated hundreds of peer-reviewed publications.

Ultra-Wide Tuning Range

The InSight platform is designed for advanced multiphoton microscopy in neuroscience, immunology, and other biological sciences. Based on patented technology1 , InSight features a broad, gap free tuning curve that spans 680 nm to 1300 nm; nearly doubling the wavelength coverage of legacy Ti:Sapphire lasers. The InSight tuning curve peaks in the 900 to 1100 nm region to excite the most common green and red fluorophores including GFP, RFP, GCaMP, jRGECO, and mCherry. The InSight X3+ provides higher powers at the longest wavelengths between 1280–1300 nm for excitation of far red fluorophores.

Exceptional Performance

With Spectra-Physics’ integrated and patented DeepSee™ dispersion pre-compensation module, short femtosecond pulses are optimally delivered through the microscope to provide maximum fluorescence and penetration depth. The InSight has exceptional beam pointing stability, beam quality, output power stability and noise, with fast wavelength tuning. When equipped with the fixed 1045 nm dual beam option, the InSight platform fully supports the diverse needs of multimodal imaging. The two synchronized output beams enable simultaneous imaging of various fluorescence proteins, genetically encoded calcium indicators, second and third harmonic generation, and advanced imaging techniques such as CARS and SRS.

InSight X3+ A and InSight X3 A Models

Built on the proven InSight platform, InSight X3+ A and InSight X3 A lasers are equipped with integrated attenuation for real-time optimization of the experimental pulse energy. Each model delivers high average power, short pulses and dispersion compensation to support the most demanding experiments in neuroscience, immunology and biology.